A Secret Weapon For attune flow cytometer
A Secret Weapon For attune flow cytometer
Blog Article
Facts-enriched characterization of mobile populations. Morphological facts from illustrations or photos can include on the richness of flow cytometry facts. For instance, the figure displays an usually regular apoptosis assay working with Annexin V and PI, introducing cell imaging to characterize cells in Every single populace to reveal morphologically distinctive functions. These insights couldn't are already obtained from multiplex staining on your own.
As samples are acquired over the Attune CytPix Flow Cytometer, the large-speed brightfield camera captures and shops pictures of detected events in a rate around six,000 visuals for each next, with regards to the flow charge and image measurement. For higher adaptability, Attune Cytometric Computer software helps you to modify the impression capture frequency as required and select a selected gate for graphic capture, if essential.
(B) RAW 264.7 macrophages are often annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The quantity of singlets available for Evaluation boosts as soon as the consumer trains and employs a more exact product.
Find out new insights into your experimental data by visualizing populations working with merged fluorescence and morphology information. Our software program interface wizards help downsampling, concatenation (merged documents) and dimensionality instruments that happen to be crafted to the application for an intuitive, streamlined data Investigation expertise.
"An image isn't really rather really worth 1000 dots, nonetheless it seriously helps you to see visuals. It is great to see photos that correlate with staining. By way of example, AnnexinV-constructive cells which are morphologically different than wholesome cells, or CD14+ monocytes which have been greater plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it truly is illuminating to see the number of doublets slip by classic singlet gates, and wonderful to verify the standing of unpredicted double-beneficial events in my Assessment."
Pair your Attune flow cytometer with the autosampler for enhanced workflow performance, and add robotic integration for max throughput.
Regular image top quality even at higher throughput Acoustic concentrating plus a substantial-speed digital camera Blend to picture these T cells persistently at small or large flow fees. Easily modify target and camera options to satisfy experimental prerequisites.
The Attune NxT cytometer will be the dependable workhorse instrument your lab can rely on for constant and trusted overall performance. The Attune CytPix product contains a significant-speed brightfield camera and AI-pushed image Examination application, with modern upgrades including machine Studying resources and an intuitive user interface, enabling Improved knowledge precision, precision, and a lot quicker insights into sample biology.
Probably most importantly, these events comprise cells of Evidently distinctive phenotypes which could lead to incorrect conclusions concerning double optimistic activities (especially in exceptional populations).
Seem waves permit exact mobile alignment, leading to Extraordinary information excellent and ~10X faster sample flow costs.
We previously shown the power of imaging Vehicle-T/Ramos cell interactions. Let’s examine just the population of greatest fascination, the double favourable occasions, to learn more. We are able to now use extended morphological parameters (listed here, circularity and skewness of intensity) to further more examine the options of such populations and refine gating on these activities, growing info robustness.
Developed-in dimensionality reduction applications amplified resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to substantiate anticipated populace resolution in lysed full human blood. User can view events from Each individual cluster for visual confirmation of cell phenotypes.
No wash/no lyse leukocyte Assessment with imaging. Cells have been obtained from 24-hour-previous blood by dilution in one mM EDTA ( Skilled UX/UI enables users to teach AI designs for expanded programs. (A) The “Coach Product” aspect provides annotation equipment and actual-time product schooling precision.
At the heart of each Attune Flow Cytometer lies our innovative acoustic focusing technologies, which utilizes seem waves to meticulously align cells inside the flow cell. This revolutionary tactic surpasses traditional hydrodynamic techniques, leading to faster processing speeds, Outstanding info high-quality, get more info and greater sample throughput.
Because the mobile line had Formerly been analyzed to the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and ended up capable to document the microbial infection at least 5 days previously.
Agilent Value Assure guarantees you at least ten years of instrument use within the day you buy. Usually, We are going to credit rating you Using the residual value of the program toward an upgraded product.
Right here, we clearly show that by gating procedures employing picture-centered quantitative parameters can distinguish interacting cells from coincident situations to extra precisely evaluate interacting cells.